HPLC-ESI-QTOF-MS/MS based phytochemical characterization: estrogenic and antioxidant effect of Mangifera indica L. cv. Kent on vaginal and bone tissue of ovariectomized rats

Abstract

The phytochemical characterization of the hydroalcoholic extract of Mangifera indica leaves L. cv. Kent (MS2) was determined by HPLC-ESI-QTOF-MS/MS. Eight metabolites were identified, these include: benzophenone derivatives and xanthones. Using ovariectomized rats (OVX), our findings suggest that EM2 exhibited an estrogenic effect by increasing the number of cornified cells in vaginal tissue. Furthermore, EM2 demonstrated a high antioxidant effect by reducing malondialdehyde (MDA) levels in OVX rat bone. We conclude that the polyphenolic compounds present in EM2 could increase the number of cornified cells and act as exogenous antioxidant agents against oxidative damage in OVX rats.